Recombinant CNN1 antibody (CF®740)

Catalog No. ABIN7650462

This anti-CNN1 antibody is a Mouse Monoclonal antibody detecting CNN1 in FACS, IHC, WB and IHC (fp). Suitable for Human and Rat.

Quick Overview for Recombinant CNN1 antibody (CF®740) (ABIN7650462)

Target: CNN1 (Calponin 1 (CNN1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNN1 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RCNN1-832

Product Details anti-CNN1 Antibody (CF®740)

Purpose: Calponin-1 (Smooth Muscle Marker) (rCNN1/832), CF740 conjugate

Characteristics: Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle. Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CNN1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA pH 7.5-8.5. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Myoepithelial cells in breast ducts

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CNN1

Target: CNN1 (Calponin 1 (CNN1))

Alternative Name: Calponin-1

Background:

Synonyms: Calponin-1, CNN1, Calponin 1 basic smooth muscle, Calponin H1 smooth muscle

Gene Symbol: CNN1

Tissue Expression: Smooth muscle

Molecular Weight: 34 kDa

Gene ID: 1264

UniProt: P51911