S100A8/A9 Complex (Calprotectin) antibody (CF®740)

Catalog No. ABIN7650472

This Mouse Monoclonal antibody specifically detects S100A8/A9 Complex (Calprotectin) in IHC, FACS, WB, IP and IHC (fp). It exhibits reactivity toward Human, Pig, Cow, Cat, Dog, Guinea Pig, Monkey, Rabbit, Rat, Mouse, Horse, Baboon and Goat.

Quick Overview for S100A8/A9 Complex (Calprotectin) antibody (CF®740) (ABIN7650472)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivity: Human, Pig, Cow, Cat, Dog, Guinea Pig, Monkey, Rabbit, Rat, Mouse, Horse, Baboon, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8/A9 Complex (Calprotectin) antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MAC387

Product Details anti-S100A8/A9 Complex (Calprotectin) Antibody (CF®740)

Purpose: Calgranulin B / MRP-8/-14 / MAC 387 / S100A8/A9 / Macrophage Ag(MAC387), CF740 conjugate

Characteristics: Calprotectin is a heteroduplex of Protein S100-A8/A9 (also called MRP-8/14, or Calgranulin A/B). This antibody recognizes the 14 kDa B component (Protein S100-A9) of the complex. Protein S100-A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. Altered expression of this protein is associated with the disease cystic fibrosis. This antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Affinity purified monocyte membrane preparation

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Tonsil, lymph node, or spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Alternative Name: Calprotectin

Background:

Synonyms: Calprotectin, Protein S100-A9, Myeloid-related protein 14, MRP-14, Calgranulin B, CAGB, Calprotectin L1H Subunit

Tissue Expression: Granulocytes|Monocytes/macrophages

Molecular Weight: 12-14 kDa (doublet)

Gene ID: 6279

Pathways: S100 Proteins