Recombinant CA9 antibody (CF®568)

Catalog No. ABIN7650484

This Rabbit Monoclonal antibody specifically detects CA9 in IHC and IHC (fp). It exhibits reactivity toward Human and Horse.

Quick Overview for Recombinant CA9 antibody (CF®568) (ABIN7650484)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Antibody Type: Recombinant Antibody

Reactivity: Human, Horse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CA9 antibody is conjugated to CF®568

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CA9-2993R

Product Details anti-CA9 Antibody (CF®568)

Purpose: Renal Cell Carcinoma (Carbonic Anhydrase IX) (CA9/2993R), CF568 conjugate

Characteristics: Carbonic anhydrase IX (carbonic anhydrase 9) is one of several carbolic anhydrases that vary in tissue distribution and localization. Carbonic anhydrases catalyze the interconversion of carbon dioxide and water into carbonic acid and bicarbonate and are found in all mammalian tissues. Carbonic anhydrase IX is a transmembrane protein that is not expressed in most healthy tissues, but is expressed in most renal cell carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CA9 protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Normal kidney or renal cell carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: Carbonic Anhydrase IX

Background:

Synonyms: Carbonic anhydrase IX, Carbonic anhydrase 9, RCC-associated antigen G250, Carbonic dehydratase, CA-IX, Membrane antigen MN

Gene Symbol: CA9

Tissue Expression: Kidney

Molecular Weight: 55 kDa

Gene ID: 768

UniProt: Q16790