CDX2 antibody

Catalog No. ABIN7650758

The Mouse Monoclonal anti-CDX2 antibody has been validated for IHC and IHC (fp). It is suitable to detect CDX2 in samples from Human.

Quick Overview for CDX2 antibody (ABIN7650758)

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDX2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PCRP-CDX2-1A3

Product Details anti-CDX2 Antibody

Purpose: CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker) (PCRP-CDX2-1A3)

Characteristics: The specificity of this monoclonal antibody to its intended target was validated by HuProt™Array, containing more than 19,000, full-length human proteins.The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CDX2protein

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Immunoprecipitation 2-4 μg/mg protein. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HT29 cells or Colon Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CDX2

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Alternative Name: CDX2

Background:

Synonyms: Caudal type homeobox 2, Caudal type homeobox transcription factor 2, Caudal-type homeobox protein 2,CDX2

Gene Symbol: CDX2

Tissue Expression: Intestine

Molecular Weight: 40 kDa

Gene ID: 1045

UniProt: Q99626

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance