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CD2 antibody (CF®740)

This anti-CD2 antibody is a Mouse Monoclonal antibody detecting CD2 in FACS and IF. Suitable for Human.
Catalog No. ABIN7650939

Quick Overview for CD2 antibody (CF®740) (ABIN7650939)

Target

See all CD2 Antibodies
CD2

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD2 antibody is conjugated to CF®740

Application

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Flow Cytometry (FACS), Immunofluorescence (IF)

Clone

BH1
  • Purpose

    CD2(BH1), CF740 conjugate

    Characteristics

    CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    Human CD2 protein

    Isotype

    IgG2b, kappa
  • Application Notes

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

    Comment

    Positive Control: 293T or Jurkat cells. Lymph node and tonsil

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.1 mg/mL

    Buffer

    PBS, 0.1 % rBSA, 0.05 % azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect from light

    Storage

    4 °C

    Storage Comment

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    Expiry Date

    24 months
  • Target

    CD2

    Alternative Name

    CD2

    Background

    Synonyms: Erythrocyte receptor, LFA-2, LFA-3 receptor, Ly-37, Lymphocyte function antigen 2, Rosette receptor, SRBC, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5, T11

    Gene Symbol: CD2

    Tissue Expression: Lymphocytes|NK cells|T-cells

    Molecular Weight

    ~50 kDa

    Gene ID

    914

    UniProt

    P06729
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