Recombinant CD3 epsilon antibody (Extracellular Domain)

Catalog No. ABIN7651254

The Mouse Monoclonal anti-CD3 epsilon antibody (Clone rC3e-1931) (ABIN7651254) specifically detects CD3 epsilon in IHC and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for Recombinant CD3 epsilon antibody (Extracellular Domain) (ABIN7651254)

Target: CD3 epsilon (CD3E)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RC3e-1931

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 23-119, Extracellular Domain

Purpose: CD3e (T-Cell Marker)(rC3e/1931)

Characteristics: The immunogen for this Mab is derived from the extracellular domain of CD3 epsilon chain. CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CD3e extracellular domain fragment (around aa 23-119)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Jurkat cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Preservative: Without preservative

Storage: -20 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background:

Synonyms: CD3 epsilon, CD3 TCR complex, T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Gene Symbol: CD3E

Tissue Expression: T-cells

Molecular Weight: 20 kDa

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway