CD44 Standard antibody (CF®740)

Catalog No. ABIN7651492

This Mouse Monoclonal antibody specifically detects CD44 Standard in FACS, IF, WB, IHC, IHC (fp) and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for CD44 Standard antibody (CF®740) (ABIN7651492)

Target: CD44 Standard (CD44s)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 Standard antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Clone: DF1485

Product Details anti-CD44 Standard Antibody (CF®740)

Purpose: CD44 Standard(DF1485), CF740 conjugate

Characteristics: Recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified CD44 antigen (PGp-1) from lymphocyte membrane

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa cells or paracortex in tonsil or lymph node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD44 Standard

Target: CD44 Standard (CD44s)

Alternative Name: CD44 Standard

Background:

Synonyms: LHR, BA-1, chondroitin sulfate proteoglycan 8 (CSPG8), Epican, Extracellular Matrix Receptor III (ECM III), GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan Sulfate Proteoglycan, Hermes Antigen, HAS, HUTCH I, Hyaluronate Receptor, Indian blood group, Inlu Related p80 Glycoprotein, Ly 24, MDU2, MDU3, MIC4, MUTCH I, Phagocytic Glycoprotein 1 (PGP-1)

Gene Symbol: CD44

Molecular Weight: 80-95 kDa

Gene ID: 960

UniProt: P16070