CD44v3 antibody (CF®647)

Catalog No. ABIN7651496

The Mouse Monoclonal anti- antibody has been validated for IHC and IHC (fp). It is suitable to detect in samples from Human.

Quick Overview for CD44v3 antibody (CF®647) (ABIN7651496)

Target: CD44v3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 3G5

Product Details

Purpose: CD44v3 (Marker of Tumor Metastasis)(3G5), CF647 conjugate

Characteristics: This antibody recognizes an epitope encoded by exon v3 on the variant portion of human CD44. The CD44 Molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment corresponding to the v9 domain of human CD44

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Cervix or Tongue Squamous Cell Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: CD44v3

Background:

Synonyms: LHR, BA-1, chondroitin sulfate proteoglycan 8 (CSPG8), Epican, Extracellular Matrix Receptor III (ECM III), GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan Sulfate Proteoglycan, Hermes Antigen, HAS, HUTCH I, Hyaluronate Receptor, Indian blood group, Inlu Related p80 Glycoprotein, Ly 24, MDU2, MDU3, MIC4, MUTCH I, Phagocytic Glycoprotein 1 (PGP-1)

Gene Symbol: CD44

Molecular Weight: 80-95 kDa

Gene ID: 960

UniProt: P16070