CD48 antibody (CF®740)

Catalog No. ABIN7651649

The Mouse Monoclonal anti-CD48 antibody has been validated for FACS, IF and Func. It is suitable to detect CD48 in samples from Human.

Quick Overview for CD48 antibody (CF®740) (ABIN7651649)

Target: CD48

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD48 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: 156-4H9

Product Details anti-CD48 Antibody (CF®740)

Purpose: CD48(156-4H9), CF740 conjugate

Characteristics: Reacts with human CD48, a 45 kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human CD48 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Daudi, JY, Raji, Jurkat, and human lymphocytes. Human lymph node and tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD48

Target: CD48

Alternative Name: CD48

Background:

Synonyms: B-cell activation marker|B-lymphocyte activation marker BLAST-1|BCM1 surface antigen|BLAST1|CD48 antigen (B cell membrane protein)|Signaling lymphocytic activation molecule 2 (SLAMF2)|SLAM family member 2

Gene Symbol: CD48

Tissue Expression: Lymphocytes|Monocytes/macrophages

Molecular Weight: 45 kDa

Gene ID: 962

UniProt: P09326