Recombinant CD52 antibody (CF®640R)

Catalog No. ABIN7651696

This Rabbit Monoclonal antibody specifically detects CD52 in IHC and IHC (fp). It exhibits reactivity toward Human and Monkey.

Quick Overview for Recombinant CD52 antibody (CF®640R) (ABIN7651696)

Target: CD52

Antibody Type: Recombinant Antibody

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD52 antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CD52-2276R

Product Details anti-CD52 Antibody (CF®640R)

Purpose: CD52 (Epididymis-Specific Protein 5) (CD52/2276R), CF640R conjugate

Characteristics: Human CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CD52 protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Human peripheral blood cells, Daudi cell lines or human tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD52

Target: CD52

Alternative Name: CD52

Background:

Synonyms: Cambridge pathology 1 antigen, CAMPATH 1, CAMPATH 1 antigen, CD52, CD52 antigen, CDw52, CDW52 antigen, Epididymal secretory protein E5, Epididymis secretory sperm binding protein Li 171mP, He5, Human epididymis-specific protein 5

Gene Symbol: CD52

Molecular Weight: 6 kDa

Gene ID: 1043

UniProt: P31358