CD53 antibody (CF®740)

Catalog No. ABIN7651706

The Mouse Monoclonal anti-CD53 antibody has been validated for FACS, IP and Func. It is suitable to detect CD53 in samples from Human.

Quick Overview for CD53 antibody (CF®740) (ABIN7651706)

Target: CD53

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD53 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Clone: 63-5A3

Product Details anti-CD53 Antibody (CF®740)

Purpose: CD53(63-5A3), CF740 conjugate

Characteristics: Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop V, Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human Sezary cells

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD53

Target: CD53

Alternative Name: CD53

Background:

Synonyms: CD53 glycoprotein, CD53 tetraspan antigen, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, Tetraspanin-25 (Tspan-25), TSPAN25

Gene Symbol: CD53

Tissue Expression: Hematopoietic cells|Osteoblasts/osteoclasts

Molecular Weight: 33-55 kDa

Gene ID: 963

UniProt: P19397