CD6 antibody

Catalog No. ABIN7651738

This Mouse Monoclonal antibody specifically detects CD6 in FACS, IF and Func. It exhibits reactivity toward Human and Mouse.

Quick Overview for CD6 antibody (ABIN7651738)

Target: CD6

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD6 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: SPV-L14

Product Details anti-CD6 Antibody

Purpose: CD6 (Negative Marker of T-regulatory Cells)(SPV-L14)

Characteristics: CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Immunogen: CD8 cytotoxic T-cell clone

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: 1-2 μg/mL. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: CCRF-CEM, Jurkat cells, Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD6

Target: CD6

Alternative Name: CD6

Background:

Synonyms: T12, TP120

Gene Symbol: CD6

Tissue Expression: B-cells|T-cells

Molecular Weight: 90-130 kDa

Gene ID: 923

UniProt: P30203