CD63 antibody (Biotin)

Catalog No. ABIN7651801

This Mouse Monoclonal antibody specifically detects CD63 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD63 antibody (Biotin) (ABIN7651801)

Target: CD63

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: LAMP3-529

Product Details anti-CD63 Antibody (Biotin)

Purpose: CD63 (Late Endosomes Marker)(LAMP3/529), Biotin conjugate

Characteristics: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Immunogen: Recombinant human CD63 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Flow cytometry: 1-2 μg/million cells. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: For coating order antibody without BSA. Immunofluorescence: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: U87MG, SK-MEL-28, HL60, THP-1, NIH/3T3, or MCF-7 cells. Human melanoma, spleen or lymphoma tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD63

Target: CD63

Alternative Name: CD63

Background:

Synonyms: gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin-30, TSPAN30

Gene Symbol: CD63

Tissue Expression: Platelets|Granulocytes|Lymphocytes|Monocytes/macrophages

Molecular Weight: 26 kDa (core protein), 30-60 kDa (glycosylated)

Gene ID: 967

UniProt: P08962