CD68 antibody (AA 150-221) (CF®594)

Catalog No. ABIN7651904

The Mouse Monoclonal anti-CD68 antibody has been validated for IHC and IHC (fp). It is suitable to detect CD68 in samples from Human.

Quick Overview for CD68 antibody (AA 150-221) (CF®594) (ABIN7651904)

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD68 antibody is conjugated to CF®594

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: C68-2501

Product Details anti-CD68 Antibody (CF®594)

Binding Specificity: AA 150-221

Purpose: CD68 (Macrophage Marker) (C68/2501), CF594 conjugate

Characteristics: This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human CD68 protein (around aa 150-221)

Isotype: IgG2c, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Western: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Tonsil, lymph node, or spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68

Background:

Synonyms: GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1

Gene Symbol: CD68

Tissue Expression: Monocytes/macrophages

Molecular Weight: ~110 kDa

Gene ID: 968

UniProt: P34810