CD7 antibody (CF®740)

Catalog No. ABIN7651940

The Mouse Monoclonal anti-CD7 antibody has been validated for . It is suitable to detect CD7 in samples from Human.

Quick Overview for CD7 antibody (CF®740) (ABIN7651940)

Target: CD7

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD7 antibody is conjugated to CF®740

Application: Please inquire

Clone: CD7-3737

Product Details anti-CD7 Antibody (CF®740)

Purpose: CD7 (T-Cell Leukemia Marker)(CD7/3737), CF740 conjugate

Characteristics: Recognizes a protein of 40 kDa, identified as CD7 (also known as gp40, Leu9). CD7 is a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CD7 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Jurkat or Molt-4 cells. Human peripheral blood lymphocytes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD7

Target: CD7

Alternative Name: CD7

Background:

Synonyms: GP40, Leu9, p41, T-cell leukemia antigen, T-cell surface antigen Leu-9, Tp40, TP41

Gene Symbol: CD7

Tissue Expression: NK cells|T-cells

Molecular Weight: 40 kDa

Gene ID: 924

UniProt: P09564

Pathways: Cell-Cell Junction Organization