Recombinant CD81 antibody (CF®488A)

Catalog No. ABIN7652083

This Mouse Monoclonal antibody specifically detects CD81 in FACS, IHC, IF, ExoS and IHC (fp). It exhibits reactivity toward Human and Rat.

Quick Overview for Recombinant CD81 antibody (CF®488A) (ABIN7652083)

Target: CD81

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD81 antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Exosome Staining (ExoS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RC81-3442

Product Details anti-CD81 Antibody (CF®488A)

Purpose: CD81 / TAPA-1 (rC81/3442), CF488A conjugate

Characteristics: This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI, Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CD81 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes. Immunohistology (formalin): 2-4 μg/mL for 30 minutes at RT. For functional studies, order Ab without azide. Western blotting 1-2 μg/mL. Immunofluorescence 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, MCF-7, and human lymphocytes. Tonsil or liver tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD81

Target: CD81

Alternative Name: CD81

Background:

Synonyms: CD81, Target of the antiproliferative antibody 1 (TAPA-1), Tetraspanin-28, Tspan-28

Gene Symbol: CD81

Molecular Weight: 26 kDa

Gene ID: 975

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C