CD8B antibody (CF®488A)

Catalog No. ABIN7652221

This Mouse Monoclonal antibody specifically detects CD8B in . It exhibits reactivity toward Cow, Sheep and Goat.

Quick Overview for CD8B antibody (CF®488A) (ABIN7652221)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Cow, Sheep, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8B antibody is conjugated to CF®488A

Application: Please inquire

Clone: CBB-1595

Product Details anti-CD8B Antibody (CF®488A)

Purpose: CD8B (Cytotoxic / Suppressor T-Cell Marker)(CBB/1595), CF488A conjugate

Characteristics: The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Antibody was raised against bovine CD8b.

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: HuT78 or hPBL. Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8b

Background:

Synonyms: CD8 antigen 37 kDa chain, CD8 antigen, beta chain, CD8 antigen, beta polypeptide 1 (p37), CD8 antigen, beta polypeptide 1, CD8B, LEU2, LYT3, OX 8 membrane antigen, p37, T cell surface glycoprotein CD8 beta chain

Gene Symbol: CD8B

Tissue Expression: T-cells

Molecular Weight: 32 kDa

Gene ID: 926

UniProt: P10966

Pathways: TCR Signaling