CD8B antibody

Catalog No. ABIN7652228

The Rat Monoclonal anti-CD8B antibody has been validated for FACS and Func. It is suitable to detect CD8B in samples from Mouse.

Quick Overview for CD8B antibody (ABIN7652228)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8B antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: H35-17-2

Product Details anti-CD8B Antibody

Purpose: CD8b, Mouse(H35-17.2)

Characteristics: This rat monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: MPBMC or Thymus.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8b

Background:

Synonyms: CD8b antigen, CD8 antigen beta polypeptide 1 (p37), CD8 beta, CD8B1, Leu2, Ly3, LYT3, P37, T lymphocyte surface glycoprotein beta chain, T-cell surface glycoprotein CD8 beta chain

Gene Symbol: Cd8b1 (Mouse)

Tissue Expression: T-cells

Molecular Weight: 37 kDa

Gene ID: 12526

UniProt: P10300

Pathways: TCR Signaling