CD99 antibody (CF®640R)

Catalog No. ABIN7652317

This Mouse Monoclonal antibody specifically detects CD99 in . It exhibits reactivity toward Human.

Quick Overview for CD99 antibody (CF®640R) (ABIN7652317)

Target: CD99

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is conjugated to CF®640R

Application: Please inquire

Clone: 1-20E 08

Product Details anti-CD99 Antibody (CF®640R)

Purpose: CD99 / MIC2 (Ewing's Sarcoma Marker)(12E7), CF640R conjugate

Characteristics: This antibody recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.

Immunogen: Human acute lymphocytic leukemia T-cells

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: MOLT-4 cells. Pancreas or Ewing sarcoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD99

Target: CD99

Alternative Name: CD99

Background:

Synonyms: 12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2

Gene Symbol: CD99

Tissue Expression: Lymphocytes|Ovary|Pancreatic islet cells|T-cells|Testis

Molecular Weight: 27-32 kDa

Gene ID: 4267

UniProt: P14209