Recombinant CD99 antibody (CF®740)

Catalog No. ABIN7652329

This Rabbit Monoclonal antibody specifically detects CD99 in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Recombinant CD99 antibody (CF®740) (ABIN7652329)

Target: CD99

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD99 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MIC2-1495R

Product Details anti-CD99 Antibody (CF®740)

Purpose: CD99 / MIC2 (Ewing s Sarcoma Marker) (MIC2/1495R), CF740 conjugate

Characteristics: This antibody recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length MIC2 protein

Isotype: IgG, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 2-4 μg/mL. Flow cytometry 2-4 μg/million cells. Immunohistology (formalin) 5-10 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: MOLT-4 cells. Pancreas or Ewing s sarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD99

Target: CD99

Alternative Name: CD99

Background:

Synonyms: 12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2

Gene Symbol: CD99

Tissue Expression: Lymphocytes|Ovary|Pancreatic islet cells|T-cells|Testis

Molecular Weight: 27-32 kDa

Gene ID: 4267

UniProt: P14209