CDw78 antibody (CF®568)
Quick Overview for CDw78 antibody (CF®568) (ABIN7652349)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- CDw78(DF1588), CF568 conjugate
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Characteristics
- This antibody reacts with a carbohydrate determinant, identified as CDw78 (Workshop V, Code CDw78.2). Epitope recognized by this MAb and Leu21 is the same. It is present on some immature and some peripheral blood B cells, and some B cell lines. Its expression is increased after B-cell activation in vitro. CDw78 is also found on tissue macrophages and on epithelial cells, but not on T cells, NK cells, monocytes, granulocytes and myeloid tissues. It appears that CDw78 is a determinant on a major histocompatibility complex (MHC) class II subpopulation.
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Immunogen
- Human leucocytes
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Isotype
- IgG1, kappa
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Application Notes
- For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry
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Comment
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Positive Control: Raji and human peripheral blood lymphocytes. Human tonsil and lymph node.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.1 mg/mL
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Buffer
- PBS, 0.1 % BSA, 0.05 % azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Protect from light
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Storage
- 4 °C
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Storage Comment
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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Expiry Date
- 24 months
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- CDw78
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Background
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Synonyms: Leu21, Ba antigen, LO panBa
Gene Symbol: Not Known
Tissue Expression: Macrophage/monocyte|B-cells|Epithelial cells
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Molecular Weight
- Not Known
Target
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