CDC34 antibody (CF®405S)

Catalog No. ABIN7652358

This anti-CDC34 antibody is a Mouse Monoclonal antibody detecting CDC34 in IHC and IHC (fp). Suitable for Human.

Quick Overview for CDC34 antibody (CF®405S) (ABIN7652358)

Target: CDC34 (Cell Division Cycle 34 (CDC34))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC34 antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CPTC-CDC34-2

Product Details anti-CDC34 Antibody (CF®405S)

Purpose: Cell Division Cycle 34 homolog(CPTC-CDC34-2), CF405S conjugate

Characteristics: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CDC34 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: 293, K562, Jurkat cell lysates. Human small intestine.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CDC34

Target: CDC34 (Cell Division Cycle 34 (CDC34))

Alternative Name: Cell Division Cycle 34 homolog

Background:

Synonyms: Cdc34, cell division cycle 34, UBC3, UBCH3, UBE2R1, Ubiquitin carrier protein, Ubiquitin conjugating enzyme E2 32 kDa complementing, Ubiquitin protein ligase R1, Ubiquitin conjugating enzyme E2-CDC34

Gene Symbol: CDC34

Molecular Weight: 34 kDa

Gene ID: 997

UniProt: P49427