Chromogranin A antibody (CF®488A)

Catalog No. ABIN7652423

The Rabbit Polyclonal anti-Chromogranin A antibody has been validated for IHC and IHC (fp). It is suitable to detect Chromogranin A in samples from Human.

Quick Overview for Chromogranin A antibody (CF®488A) (ABIN7652423)

Target: Chromogranin A (CHGA)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chromogranin A antibody is conjugated to CF®488A

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Chromogranin A Antibody (CF®488A)

Purpose: Chromogranin A / CHGA (Neuroendocrine Marker), CF488A conjugate

Characteristics: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human chromogranin A protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: PC12 cells. Adrenal gland, bowel, parathyroid, pancreas, or pheochromocytoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: CHGA

Background:

Synonyms: Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, Vasostatin I or II

Gene Symbol: CHGA

Tissue Expression: Neuroendocrine cells

Molecular Weight: 68-75 kDa

Gene ID: 1113

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling