F13A1 antibody (AA 46-181) (CF®740)

Catalog No. ABIN7652470

This Mouse Monoclonal antibody specifically detects F13A1 in WB, IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for F13A1 antibody (AA 46-181) (CF®740) (ABIN7652470)

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This F13A1 antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: F13A1-1448

Product Details anti-F13A1 Antibody (CF®740)

Binding Specificity: AA 46-181

Purpose: Factor XIIIa (Coagulation Factor XIIIA Chain) (F13A1/1448), CF740 conjugate

Characteristics: This antibody recognizes a protein of 83 kDa, which is identified as Factor XIIIa. It has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/ ) and desmoplastic malignant melanoma (-). Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human Factor XIIIa protein (aa46-181)

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: 2-4 μg/mL for coating order Ab without BSA. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: 293T Cells. Placenta.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for F13A1

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Alternative Name: Factor-XIIIa

Background:

Synonyms: Coagulation factor XIII A chain, Coagulation factor XIII A1 polypeptide, Coagulation factor XIIIa, F13A, F13a1, Factor XIIIA, Fibrin stabilizing factor, A subunit, Fibrinoligase, FSF, A subunit, Protein-glutamine gamma-glutamyltransferase A chain, TGase, Transglutaminase A chain, Transglutaminase. plasma

Gene Symbol: F13A1

Tissue Expression: Platelets|Uterus|Megakaryocytes|Monocytes/macrophages|Placenta|Prostate

Molecular Weight: 83 kDa

Gene ID: 2162

UniProt: P00488