Recombinant G-CSF antibody (CF®740)

Catalog No. ABIN7652499

This anti-G-CSF antibody is a Mouse Monoclonal antibody detecting G-CSF in IHC and IHC (fp). Suitable for Human and Macaque.

Quick Overview for Recombinant G-CSF antibody (CF®740) (ABIN7652499)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Antibody Type: Recombinant Antibody

Reactivity: Human, Macaque

Host: Mouse

Clonality: Monoclonal

Conjugate: This G-CSF antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RCSF3-900

Product Details anti-G-CSF Antibody (CF®740)

Purpose: Granulocyte-Colony Stimulating Factor (G-CSF)(rCSF3/900), CF740 conjugate

Characteristics: This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73 % sequence identity at the amino acid level. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CSF3 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Tonsil or lymph node (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: Granulocyte Colony Stimulating Factor

Background:

Synonyms: Pluripoietin, Filgrastim, Lenograstim, CSF3OS, G-CSF, chromosome 17 open reading frame 33 (C17orf33), colony stimulating factor 3 (granulocyte)

Gene Symbol: CSF3

Tissue Expression: Granulocytes|Myeloid cells

Molecular Weight: 19 kDa

Gene ID: 1440

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization