CPK antibody (Phosphospecific) (CF®740)

Catalog No. ABIN7652699

The Mouse Monoclonal anti-CPK antibody has been validated for . It is suitable to detect CPK in samples from Human.

Quick Overview for CPK antibody (Phosphospecific) (CF®740) (ABIN7652699)

Target: CPK (Creatine Phosphokinase (CPK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CPK antibody is conjugated to CF®740

Application: Please inquire

Clone: 2ba6

Product Details anti-CPK Antibody (CF®740)

Binding Specificity: Phosphospecific

Purpose: Creatine Phosphokinase(2ba6), CF740 conjugate

Characteristics: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments. They provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteinsPrimary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human CKBB protein

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Not suitable for FFPE sections. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Cerebellum

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CPK

Target: CPK (Creatine Phosphokinase (CPK))

Alternative Name: Creatine Phosphokinase

Background:

Synonyms: B-CK, BB-CK, creatine kinase-B, Creatine kinase B chain, CKBB creatine kinase B-type, creatine kinase, brain, CKBB, Brain creatine kinase, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine kinase brain, Creatine phosphokinase BB

Gene Symbol: CKB

Molecular Weight: 43 kDa (Monomer), 86 kDa (Dimer)

Gene ID: 1152

UniProt: P12277