Cyclin A1 antibody (CF®568)

Catalog No. ABIN7652713

This Mouse Monoclonal antibody specifically detects Cyclin A1 in WB. It exhibits reactivity toward Xenopus laevis.

Quick Overview for Cyclin A1 antibody (CF®568) (ABIN7652713)

Target: Cyclin A1 (CCNA1)

Reactivity: Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin A1 antibody is conjugated to CF®568

Application: Western Blotting (WB)

Clone: XLA1-3

Product Details anti-Cyclin A1 Antibody (CF®568)

Purpose: Cyclin A1(XLA1-3), CF568 conjugate

Characteristics: The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. The antibody detects Xenopus cyclin A in blots. Cyclin A1 is the embryonic cyclin which is present in Xenopus eggs. This MAb only detects Xenopus Cyclin A1. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length Xenopus laevis Cyclin A1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: Very high levels in testis. Also found in myeloid leukemia cell lines. Xenopus laevis tissue lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cyclin A1

Target: Cyclin A1 (CCNA1)

Alternative Name: Cyclin A1

Background:

Synonyms: CCN1, CCNA, CCNA2, Cyclin A1

Gene Symbol: ccna1

Molecular Weight: 65 kDa

UniProt: P18606

Pathways: Apoptosis, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA