Cytochrome P450 antibody (C-Term) (CF®594)

Catalog No. ABIN7653010

This Mouse Monoclonal antibody specifically detects Cytochrome P450 in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Cytochrome P450 antibody (C-Term) (CF®594) (ABIN7653010)

Target: Cytochrome P450 (CYP)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome P450 antibody is conjugated to CF®594

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: M12P4H2

Product Details anti-Cytochrome P450 Antibody (CF®594)

Binding Specificity: C-Term

Purpose: Cytochrome p450(M12P4H2), CF594 conjugate

Characteristics: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases, which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines, which are pre-mutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Ovalbumin-conjugated synthetic peptide HIGFGCIPPR (C-terminal sequence)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HepG2 cells. Liver.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cytochrome P450

Target: Cytochrome P450 (CYP)

Alternative Name: Cytochrome p450

Background:

Synonyms: 4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 , Cytochrome P450-J, EC 1.14.13, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase

Gene Symbol: CYP2E1

Molecular Weight: ~50-55 kDa

Gene ID: 1571

UniProt: P05181

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process