Recombinant Desmocollin 2+3 antibody

Catalog No. ABIN7653102

The Mouse Monoclonal anti-Desmocollin 2+3 antibody (Clone rDSC2-3437) (ABIN7653102) specifically detects Desmocollin 2+3. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant Desmocollin 2+3 antibody (ABIN7653102)

Target: Desmocollin 2+3

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Please inquire

Clone: RDSC2-3437

Product Details

Purpose: Desmocollin-2/3(rDSC2/3437)

Characteristics: Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human DSC2 protein

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: HeLa cells. Skin tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Preservative: Without preservative

Storage: -20 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Desmocollin 2+3

Alternative Name: Desmocollin-2/3

Background:

Synonyms: ARVD11, Cadherin family member 2, CDHF2, Desmocollin-2, Desmocollin-3, Desmosomal glycoprotein II and III, Desmosomal glycoprotein II, Desmosomal glycoprotein III, DG2, DGII/III, DSC2, DSC3

Gene Symbol: DSC2

Tissue Expression: Epidermal cells

Molecular Weight: 110 kDa (DSC2), 100 kDa (DSC3)

Gene ID: 1824

UniProt: Q02487