Desmoglein 1 antibody (Biotin)

Catalog No. ABIN7653113

The Mouse Monoclonal anti-Desmoglein 1 antibody has been validated for IHC and IHC (fp). It is suitable to detect Desmoglein 1 in samples from Human.

Quick Overview for Desmoglein 1 antibody (Biotin) (ABIN7653113)

Target: Desmoglein 1 (DSG1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 1 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 32-2B

Product Details anti-Desmoglein 1 Antibody (Biotin)

Purpose: Desmoglein-1 (DSG1)(32-2B), Biotin conjugate

Characteristics: Recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Bladder, skin or melanoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Desmoglein 1

Target: Desmoglein 1 (DSG1)

Alternative Name: Desmoglein-1

Background:

Synonyms: Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1

Gene Symbol: DSG1

Tissue Expression: Epithelial cells

Molecular Weight: 150 kDa (precursor), 160 kDa (mature)

Gene ID: 1828

UniProt: Q02413

Pathways: Cell-Cell Junction Organization