Dystrophin antibody (AA 114-263) (CF®647)

Catalog No. ABIN7653316

This Mouse Monoclonal antibody specifically detects Dystrophin in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Dystrophin antibody (AA 114-263) (CF®647) (ABIN7653316)

Target: Dystrophin (DMD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dystrophin antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: DMD-3243

Product Details anti-Dystrophin Antibody (CF®647)

Binding Specificity: AA 114-263

Purpose: Dystrophin (DMD) (Marker of Duchenne and Becker Muscular Dystrophy)(DMD/3243), CF647 conjugate

Characteristics: Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2. 4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least 8 independent tissue-specific promoters and 2 poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0. 002 % of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A recombinant fragment (around aa 114-263) of human DMD protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Human skeletal muscle and heart muscle tissues (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Dystrophin

Target: Dystrophin (DMD)

Alternative Name: Dystrophin

Background:

Synonyms: BMD, CMD3B, Duchenne muscular dystrophy (DMD), Dystrophin, Muscular dystrophy Duchenne and Becker types

Gene Symbol: DMD

Tissue Expression: Muscle

Molecular Weight: 427 kDa

Gene ID: 1756

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development