Emerin antibody (AA 56-167) (CF®647)

Catalog No. ABIN7653445

This Mouse Monoclonal antibody specifically detects Emerin in WB, IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Emerin antibody (AA 56-167) (CF®647) (ABIN7653445)

Target: Emerin (EMD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Emerin antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: EMD-2168

Product Details anti-Emerin Antibody (CF®647)

Binding Specificity: AA 56-167

Purpose: Emerin (Papillary Thyroid Carcinoma and EDMD Marker) (EMD/2168), CF647 conjugate

Characteristics: Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Emerin protein fragment (around aa 56-167)

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: K-562, HeLa or Jurkat cells. Kidney or Skin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Emerin

Target: Emerin (EMD)

Alternative Name: Emerin

Background:

Synonyms: EMD, Emerin, Emery Dreifuss muscular dystrophy (EDMD), STA

Gene Symbol: EMD

Molecular Weight: 37 kDa

Gene ID: 2010

UniProt: P50402