Recombinant ECM1 antibody

Catalog No. ABIN7653898

This Rabbit Monoclonal antibody specifically detects ECM1 in IHC and IHC (fp). It exhibits reactivity toward Human and Rat.

Quick Overview for Recombinant ECM1 antibody (ABIN7653898)

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ECM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ECM1-2889R

Product Details anti-ECM1 Antibody

Purpose: Secretory Component / ECM1 (ECM1/2889R)

Characteristics: This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human ECM1 protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Stomach, Lung, or breast tumor

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ECM1

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Alternative Name: ECM1

Background:

Synonyms: ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD

Gene Symbol: ECM1

Tissue Expression: Epithelial cells

Molecular Weight: ~80 kDa

Gene ID: 1893

UniProt: Q16610