Ezrin antibody (CF®568)

Catalog No. ABIN7653903

This anti-Ezrin antibody is a Mouse Monoclonal antibody detecting Ezrin in WB, IHC and IHC (fp). Suitable for Human.

Quick Overview for Ezrin antibody (CF®568) (ABIN7653903)

Target: Ezrin (EZR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ezrin antibody is conjugated to CF®568

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CPTC-Ezrin-1

Product Details anti-Ezrin Antibody (CF®568)

Purpose: Ezrin / p81(CPTC-Ezrin-1), CF568 conjugate

Characteristics: Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70 % homology with Ezrin and are co-expressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation. Overexpression of Ezrin predicts the poor prognosis of gastric adenocarcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length Ezrin protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: SKBr3, HeLa, HEK293, MCF-7, Jurkat, A431, MCF-7. Cerebral cortex and hippocampus.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background:

Synonyms: Villin-2, CVIL, Epididymis secretory protein Li 105, EZR, p81, VIL2, Cytovillin, Cytovillin 2, HEL S 105, DKFZp762H157

Gene Symbol: Ezrin

Molecular Weight: 87 kDa

Gene ID: 7430

UniProt: P15311

Pathways: Maintenance of Protein Location