FAF1 antibody

Catalog No. ABIN7653919

This Mouse Monoclonal antibody specifically detects FAF1 in WB, IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for FAF1 antibody (ABIN7653919)

Target: FAF1 (Fas (TNFRSF6) Associated Factor 1 (FAF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CPTC-FAF1-2

Product Details anti-FAF1 Antibody

Purpose: Fas (TNFRSF6) associated factor 1(CPTC-FAF1-2)

Characteristics: In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved 'death domain' and belonging to the TNF receptor superfamily. TRADD, FADD and RIP are FAS/TNF-RI interacting proteins that contain a death domain homologous region (DDH). TRADD (TNF-RI-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-RI via their DDH regions, while RIP associates exclusively with FAS. An additional FAS interacting protein designated FAF1, for FAS-associated protein factor-1, binds with the cytoplasmic tail of wildtype but not LPR mutant FAS. When overexpressed in cells, FAF1 enhances the efficiency of FAS-mediated apoptosis. In contrast to TRADD, FADD and RIP, FAF1 lacks a DDH and cannot induce apoptosis independently of FAS activation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length FAF1 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HEK293, MCF-7, HeLa whole cell lysate. Human testis, prostate, breast carcinoma or colon carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for FAF1

Target: FAF1 (Fas (TNFRSF6) Associated Factor 1 (FAF1))

Alternative Name: Fas associated factor 1

Background:

Synonyms: CGI-03, FAS-associated factor 1, HFAF1s, UBX domain-containing protein 12, UBX domain-containing protein 3A

Gene Symbol: FAF1

Molecular Weight: 75-80 kDa

Gene ID: 11124

UniProt: Q9UNN5

Pathways: Maintenance of Protein Location