Fc gamma RII (CD32) antibody (CF®647)

Catalog No. ABIN7653979

This Mouse Monoclonal antibody specifically detects in . It exhibits reactivity toward Human.

Quick Overview for Fc gamma RII (CD32) antibody (CF®647) (ABIN7653979)

Target: Fc gamma RII (CD32)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®647

Application: Please inquire

Clone: FCGR2A-479

Product Details

Purpose: CD32 (Fc Gamma RIIa)(FCGR2A/479), CF647 conjugate

Characteristics: This MAb binds most strongly to the IIa isoforms of CD32 (FcRII), a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i. e. , IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human FCGR2A protein

Isotype: IgG2b, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: K-562 cells. Lymph node, tonsil and placenta.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: CD32

Background:

Synonyms: CD32A, Fc fragment of IgG low affinity IIa receptor, Fc gamma RIIa, FCGR2A, FcgRII, IGFR2, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a, Ly-17, Ly-m20, LyM-1, Lymphocyte antigen 17

Gene Symbol: FCGR2A

Tissue Expression: Endothelial cells

Molecular Weight: 40 kDa

Gene ID: 2212

UniProt: P12318