FTL antibody (AA 38-165) (CF®740)

Catalog No. ABIN7654013

The Mouse Monoclonal anti-FTL antibody has been validated for WB, IHC and IHC (fp). It is suitable to detect FTL in samples from Human.

Quick Overview for FTL antibody (AA 38-165) (CF®740) (ABIN7654013)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FTL antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: FTL-1388

Product Details anti-FTL Antibody (CF®740)

Binding Specificity: AA 38-165

Purpose: Ferritin, Light Chain (FTL) (Microglia Marker) (FTL/1388), CF740 conjugate

Characteristics: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human FTL protein fragment (aa 38-165)

Isotype: IgG2a

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin) 0.1-0.2 μg/mL. Flow cytometry 0.1-0.2 μg/million cells Immunofluorescence 0.1-0.2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Alternative Name: Ferritin, Light Chain

Background:

Synonyms: Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, FTL, LFTD, NBIA3

Gene Symbol: FTL

Molecular Weight: 19-25 kDa

Gene ID: 2512

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis