GATA3 antibody (AA 357-436) (CF®647)

Catalog No. ABIN7654309

This Mouse Monoclonal antibody specifically detects GATA3 in . It exhibits reactivity toward Human.

Quick Overview for GATA3 antibody (AA 357-436) (CF®647) (ABIN7654309)

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GATA3 antibody is conjugated to CF®647

Application: Please inquire

Clone: GATA3-2441

Product Details anti-GATA3 Antibody (CF®647)

Binding Specificity: AA 357-436

Purpose: GATA-3 (Breast and Urothelial Marker)(GATA3/2441), CF647 conjugate

Characteristics: GATA-3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA-3 expression is primarily seen in breast and urothelial carcinomas. 'Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human GATA3 protein (around aa 357-436)

Isotype: IgG2b, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: MCF-7 or T47D cells. Breast or Bladder Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for GATA3

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Alternative Name: GATA-3

Background:

Synonyms: GATA3, GATA binding protein-3, GATA-binding factor 3, GATA3, HDR, HDRS, Transacting T-cell-specific transcription factor GATA-3

Gene Symbol: GATA3

Molecular Weight: ~50 kDa

Gene ID: 2625

UniProt: P23771

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation