Recombinant GFAP antibody (Biotin)

Catalog No. ABIN7654391

The Rabbit Monoclonal anti-GFAP antibody is suitable to detect GFAP in samples from Human, Rat, Mouse, Cow, Pig, Rabbit and Chicken. It has been validated for IHC, FACS and IHC (fp).

Quick Overview for Recombinant GFAP antibody (Biotin) (ABIN7654391)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse, Cow, Pig, Rabbit, Chicken

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GFAP antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ASTRO-1974R

Product Details anti-GFAP Antibody (Biotin)

Purpose: GFAP (Astrocyte & Neural Stem Cell Marker) (ASTRO/1974R), Biotin conjugate

Characteristics: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length GFAP protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background:

Synonyms: Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Gene Symbol: GFAP

Tissue Expression: Glia

Molecular Weight: ~50 kDa

Gene ID: 2670

UniProt: P14136