Recombinant GRP94 antibody (CF®568)

Catalog No. ABIN7654853

The Rabbit Monoclonal anti-GRP94 antibody has been validated for IHC, FACS and IHC (fp). It is suitable to detect GRP94 in samples from Human, Mouse, Rat, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Sheep, Horse and Xenopus laevis.

Quick Overview for Recombinant GRP94 antibody (CF®568) (ABIN7654853)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Sheep, Horse, Xenopus laevis

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GRP94 antibody is conjugated to CF®568

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: HSP90B1-3168R

Product Details anti-GRP94 Antibody (CF®568)

Purpose: GRP94 / HSP90B1(Glucose-Regulated Protein 94) (Endoplasmic Reticulum Marker)(HSP90B1/3168R), CF568 conjugate

Characteristics: Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been studied as a prognostic factor in breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human HSP90B1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HepG2, U20S cells. About 50% of breast tumors express grp94 protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: GRP94

Background:

Synonyms: Glucose Regulated Protein 94, 94 kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90 kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96)

Gene Symbol: HSP90B1

Molecular Weight: 94 kDa

Gene ID: 7184

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades