ATRX antibody (CF®640R)

Catalog No. ABIN7654864

This Mouse Monoclonal antibody specifically detects ATRX in . It exhibits reactivity toward Human.

Quick Overview for ATRX antibody (CF®640R) (ABIN7654864)

Target: ATRX (helicase 2, X-linked (ATRX))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATRX antibody is conjugated to CF®640R

Application: Please inquire

Clone: 23c

Product Details anti-ATRX Antibody (CF®640R)

Purpose: ATRX / RAD54 (Alpha Thalassemia Mental Retardation)(23c), CF640R conjugate

Characteristics: ATRX is a member of the Snf2 family of helicase/ATPases, which contribute to the remodeling of the nucelosome structure in an ATP-dependent manner, and facilitate the initiation of transcription and replication. Structurally, ATRX contains a PHD zinc finger motif. ATRX is regulated throughout the cell cycle where it is differentially distributed within the nucleus. During interphase, ATRX predominately associates with the nuclear matrix, while during mitosis, ATRX localizes with condensed chromatin. At the onset of M phase, phosphorylation rapidly induces this redistribution of ATRX to the short arms of human acrocentric chromosomes, where it then specifically complexes with heterochromatin protein 1 thalassemia or ATRX syndrome. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human ATRX protein

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Raji, HeLa or A431 Cells. Pancreas, Adrenal, Ovary, Kidney, Prostate or Stomach.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ATRX

Target: ATRX (helicase 2, X-linked (ATRX))

Alternative Name: ATRX

Background:

Synonyms: Transcriptional regulator ATRX, ATP-dependent helicase ATRX, RAD54 Homolog, X-linked helicase II, X-linked nuclear protein

Gene Symbol: ATRX

Molecular Weight: 280 kDa

Gene ID: 546

UniProt: P46100