Human Papilloma Virus 16 E2 (HPV-16 E2) antibody (CF®647)

Catalog No. ABIN7655166

The CF®647-conjugated Mouse Monoclonal anti-Human Papilloma Virus 16 E2 antibody (Clone TVG 261) (ABIN7655166) specifically detects Human Papilloma Virus 16 E2 in WB and IF. The antibody is reactive with Human Papillomavirus 16 (HPV-16) samples.

Quick Overview for Human Papilloma Virus 16 E2 (HPV-16 E2) antibody (CF®647) (ABIN7655166)

Target: Human Papilloma Virus 16 E2 (HPV-16 E2)

Reactivity: Human Papillomavirus 16 (HPV-16)

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®647

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: TVG 261

Product Details

Purpose: HPV16 E2 (Human Papilloma Virus 16)(TVG 261), CF647 conjugate

Characteristics: The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a strain that is responsible for the largest number of cases of cervical cancers linked to the family. HPV16E1 and HPV16E2 are proteins that are involved in the regulation of viral DNA replication and are important for infected cell homeostasis. HPV16E2 specifically regulates the expression of the E6 and E7 oncoproteins by binding to four sites within the viral long control region, possibly involving interactions with nuclear hormone receptors. Integration of the HPV genome into the host DNA usually disrupts the HPV16E2 gene open reading frames, resulting in an overexpression of E6 and E7 genes, an event that may lead to the malignant transformation of cervical cancer. HPV16E2 is also able to induce apoptotic cell death via two pathways: the first through the binding of p53 and the second through the binding of the viral genome. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Raised against Vaccinia-E2 followed by intravenous injection of the maltose binding protein MBP-E2

Isotype: IgG1, kappa

Application Details

Application Notes: Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: HPV-16 infected cells. Cervical tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Human Papilloma Virus 16 E2 (HPV-16 E2)

Alternative Name: HPV16 E2

Target Type: Viral Protein

Background:

Synonyms: HPV16 regulatory protein E2, Human papilloma virus 16 E2, Human papilloma virus 16 regulatory protein E2, Human papillomavirus type 16 E2, Human papillomavirus type 16 regulatory protein E2, Regulatory protein E2

Gene Symbol: Not Applicable

Molecular Weight: 43 kDa