ICOSLG antibody (AA 23-149) (CF®640R)

Catalog No. ABIN7655266

This Mouse Monoclonal antibody specifically detects ICOSLG in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for ICOSLG antibody (AA 23-149) (CF®640R) (ABIN7655266)

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICOSLG antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ICOSL-3260

Product Details anti-ICOSLG Antibody (CF®640R)

Binding Specificity: AA 23-149

Purpose: ICOS-L / ICOS Ligand / B7RP-1 (Immuno-Oncology Target)(ICOSL/3260), CF640R conjugate

Characteristics: This MAb recognizes a protein of 36 kDa, identified as ICOS-L. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2. 4, 3. 0, and 7. 0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1. 1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human ICOSLG (B7RP-1) protein (around aa 23-149)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HL60, HuT78 and HUVEC cells. Prostate or Colon Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Alternative Name: B7RP-1

Background:

Synonyms: B7 homolog 2 (B7H2), B7 like protein Gl50, B7 related protein 1 (B7RP1), CD275, CD275 antigen, GL50, ICOS LG, ICOS ligand (ICOSL), Inducible T cell co stimulator ligand, LICOS, Transmembrane protein B7 H2 ICOS ligand

Gene Symbol: ICOSLG

Tissue Expression: Myeloid cells

Molecular Weight: 36 kDa

Gene ID: 23308

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation