Recombinant ISR-alpha antibody (Extracellular Domain)

Catalog No. ABIN7655335

This Rabbit Monoclonal antibody specifically detects ISR-alpha in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Recombinant ISR-alpha antibody (Extracellular Domain) (ABIN7655335)

Target: ISR-alpha (Insulin Receptor alpha (ISR-alpha))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ISR-alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: INSR-2277R

Product Details anti-ISR-alpha Antibody

Binding Specificity: Extracellular Domain

Purpose: Insulin Receptor Alpha (INSR/2277R)

Characteristics: The insulin receptor (INSR) is a heterodimeric protein complex that has an intracellular beta subunit and an extracellular alpha subunit, which is disulfide-linked to a transmembrane segment. The insulin ligand binds to the INSR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to INSR induces phosphorylation of intra-cellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Type 1 diabetes is an autoimmune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of extracellular domain of human Insulin Receptor alpha

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Jurkat cells. Human pancreas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ISR-alpha

Target: ISR-alpha (Insulin Receptor alpha (ISR-alpha))

Alternative Name: Insulin Receptor Alpha

Background:

Synonyms: CD220, HHF5, HIR A, INSR, Insulin receptor, Insulin receptor subunit alpha, IR

Gene Symbol: INSR

Molecular Weight: 135 kDa

Gene ID: 3643

UniProt: P06213