CD51 antibody (CF®488A)

Catalog No. ABIN7655403

This anti-CD51 antibody is a Mouse Monoclonal antibody detecting CD51 in WB, IHC, FACS, IP, IF and Func. Suitable for Human and Chicken.

Quick Overview for CD51 antibody (CF®488A) (ABIN7655403)

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reactivity: Human, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD51 antibody is conjugated to CF®488A

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Functional Studies (Func)

Clone: 23C6

Product Details anti-CD51 Antibody (CF®488A)

Purpose: Vitronectin Receptor / CD51 / CD61(23C6), CF488A conjugate

Characteristics: ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1, 3, 5, 6, and 8, 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands, the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin Receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. Alternative splicing results in multiple transcript variants. Reacts with Integrin Alpha V Beta 3. 23C6 may be useful for bone resorption modulation, osteoclast identification, receptor purification, malignant melanoma identification and treatment. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Osteoclasts from osteoclastomas

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: For coating, order antibody without BSA, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Squamous cells, A375M melanoma cells. A549 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD51

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Alternative Name: CD51

Background:

Synonyms: CD51, DKFZp686A08142, integrin alpha V beta 3, Integrin alpha-5 Integrin alpha-V light chain, integrin alphaVbeta3, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), ITGAV, Msk8, VNRA, VTNR

Gene Symbol: ITGAV

Molecular Weight: 150-160 kDa under non-reducing conditions. Under reducing conditions, it is cleaved into 2 bands of about 125-130 kDa 20-25 kDa.

Gene ID: 3685

UniProt: P06756

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex