Integrin beta 4 antibody (CF®740)

Catalog No. ABIN7655479

This Mouse Monoclonal antibody specifically detects Integrin beta 4 in FACS, IP, IF and Func. It exhibits reactivity toward Human.

Quick Overview for Integrin beta 4 antibody (CF®740) (ABIN7655479)

Target: Integrin beta 4 (ITGB4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 4 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Functional Studies (Func)

Clone: UM-A9

Product Details anti-Integrin beta 4 Antibody (CF®740)

Purpose: CD104(UM-A9), CF740 conjugate

Characteristics: CD104 is beta 4 integrin. It is primarily expressed in epithelial cells. Together with alpha 6 integrin, it is the cell surface receptor for laminin, where it mediates cell adhesion. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human squamous cell carcinoma (UM-SCC1)

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Biological blockade partially blocks laminin binding. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: A431 cells, squamous cell carcinoma, colon or placenta

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: CD104

Background:

Synonyms: CD104, Integrin beta 4, ITGB4, GP150

Gene Symbol: ITGB4

Tissue Expression: Epithelial cells

Molecular Weight: 205 kDa

Gene ID: 3691

UniProt: P16144

Pathways: Integrin Complex