ICAM1 antibody (CF®740)

Catalog No. ABIN7655541

This anti-ICAM1 antibody is a Mouse Monoclonal antibody detecting ICAM1 in IHC and IHC (fp). Suitable for Human.

Quick Overview for ICAM1 antibody (CF®740) (ABIN7655541)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: W-CAM-1

Product Details anti-ICAM1 Antibody (CF®740)

Purpose: CD54(W-CAM-1, same as Wehi-CAM-1 or 1H4), CF740 conjugate

Characteristics: Recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Raji Burkitt lymphoma cells

Isotype: IgG2b, kappa

Application Details

Application Notes: Immunohistology formalin-fixed 2-4 ug/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Immunofluorescence 0.5-1 ug/mL. Flow Cytometry 0.5-1 ug/million cells/0.1 mL. Functional Studies order Ab without BSA & azide). Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54

Background:

Synonyms: Intestinal mucin-3A, intestinal mucin-like, MUC3, MUC3A, MUC3B, mucin 3, intestinal, mucin 3A, cell surface associated, mucin 3A, intestinal, mucin 3B, cell surface associated

Gene Symbol: ICAM1

Tissue Expression: Endothelial cells|Lymphocytes|Monocytes/macrophages

Molecular Weight: 85-115 kDa

Gene ID: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling