ICAM1 antibody (Biotin)

Catalog No. ABIN7655549

The Mouse Monoclonal anti-ICAM1 antibody has been validated for FACS, IHC, IF and Func. It is suitable to detect ICAM1 in samples from Human and Pig.

Quick Overview for ICAM1 antibody (Biotin) (ABIN7655549)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func)

Clone: 15-2

Product Details anti-ICAM1 Antibody (Biotin)

Purpose: CD54 / ICAM-1(15.2), Biotin conjugate

Characteristics: Recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. ICAM-1 may also be related to progression and metastasis of tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human monocytes.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54

Background:

Synonyms: CD54, Cell surface glycoprotein P3.58, Human rhinovirus receptor, ICAM-1, Intercellular adhesion molecule 1, Ly 47, Major group rhinovirus receptor, MALA2, MyD10, Surface antigen of activated B cells

Gene Symbol: ICAM1

Tissue Expression: Endothelial cells|Lymphocytes|Monocytes/macrophages

Molecular Weight: 85-115 kDa

Gene ID: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling