ICAM-3/CD50 antibody (CF®405S)

Catalog No. ABIN7655554

This Mouse Monoclonal antibody specifically detects ICAM-3/CD50 in FACS and Func. It exhibits reactivity toward Human.

Quick Overview for ICAM-3/CD50 antibody (CF®405S) (ABIN7655554)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM-3/CD50 antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: ICO-60

Product Details anti-ICAM-3/CD50 Antibody (CF®405S)

Purpose: CD50 / ICAM3(ICO-60), CF405S conjugate

Characteristics: Recognizes the N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C.

Immunogen: Human ICAM3

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: CD50

Background:

Synonyms: ICAMR, Intercellular adhesion molecule 3 (ICAM3)

Gene Symbol: ICAM3

Tissue Expression: Lymphocytes|T-cells

Molecular Weight: 110-160 kDa

Gene ID: 3385

UniProt: P32942