Involucrin antibody (CF®740)

Catalog No. ABIN7655751

This anti-Involucrin antibody is a Mouse Monoclonal antibody detecting Involucrin in IHC and IHC (fp). Suitable for Human, Dog, Pig, Gorilla, Monkey and Owl.

Quick Overview for Involucrin antibody (CF®740) (ABIN7655751)

Target: Involucrin (IVL)

Reactivity: Human, Dog, Pig, Gorilla, Monkey, Owl

Host: Mouse

Clonality: Monoclonal

Conjugate: This Involucrin antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: SY5

Product Details anti-Involucrin Antibody (CF®740)

Purpose: Involucrin(SY5), CF740 conjugate

Characteristics: This antibody recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified involucrin from human keratinocytes

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.1-0.2 μg/mL. Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37C, enzyme digestion is better than citrate buffer based epitope unmasking. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence. Does not react with mouse, others not known. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Involucrin

Target: Involucrin (IVL)

Alternative Name: Involucrin

Background:

Synonyms: IVL

Gene Symbol: IVL

Molecular Weight: 66-170 kDa

Gene ID: 3713

UniProt: P07476